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;SSR>RAPD>RFLP,whichhasbeeninternationallyrecognizedinthe92thASHSconference[17].ThisreviewsummarizestherecentdevelopmentofbothSSRandAFLPmarkertechnologyforvarietydifferentiation.

2.1SSRmarker

EST-SSRmolecularmarkertechniquewasconductedbyZhaoetc[18]toidentify12Chinesecabbagecultivars.Basedonexpressedsequencetags（ESTs）ofChinesecabbageinGenBank,30pairsofscreenedSSRprimersweredesignedandsynthesized,resultingin21pairsofEST-SSRprimerswhichwereeffectivelyamplified,butonly10pairsofEST-SSRprimerswerehighlypolymorphic.Accordingtotheidentificationresultsandthemappingdifference,10pairsofprimerswithhighpolymorphismweredesignedas2setsofmultiplexEST-SSRmarkerstodistinguishthese12Chinesecabbagevarieties,withsatisfactorypolymorphicrateof88.9%and97.0%respectively,aswellashighpolymorphisminformationcontentof0.910%.Laietc[19]selected26inbredlinesand54testvarietiesfortheexaminationofdistinctness,uniformityandstability（DUS）ofthesevarietiesbyadoptingSSRmarkers.49pairsofSSRprimerswerescreenedfrom952pairsintotal,basedonthecriteriaofrichnessofpolymorphisminformationcontent（PIC）,theclearnessofPCRbandsandconvenienceofdifferentalleleidentification.49pairsofSSRprimersledto57lociwith311allelesidentifiedintotal.Theaveragenumberofallelesperlocuswas5.5,rangingfrom2to13,withameanPICof0.53.Clusteranalysisshowedthatalltestvarietieswereclearlydistinguishedby49markerswhenthegeicsimilaritycoefficientwassetas0.93.

保护遗传学方法在生物多样性监测和评价领域的应用参考属性评定
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Inordertoproviderobustreferencefortheidentificationofbarleyvarietiesandavoidcounterfeitandinferiorvarieties,Wangetc[20]selected29barleystandardvarietiesandgeicdiversitywasanalyzedbyDUStesting.28pairsofhighlypolymorphicSSRprimerswerechosen,leadingto125allelesmeasuredintotal.Eachpairofpolymorphicprimersdetectedanaverageof4.46alleles,withpolymorphisminformationcontent（PIC）varyingfrom0.81to0.25andanaveragePICof0.62among28pairs.

Thespecificityandstabilityof123representativericevarietieswereanalyzedbyTianect[21]basedonSSRfingerprintingprofiles,andthevalueofSSRcoremarkerschoseninthisstudywasexamined.24pairsofprimersdetected138allelesintotal,with12locidetectedinsinglecultivarand21locisuccessfullydistinguishingjaponicaandindicaricevarieties.Onthebasisofgeicsimilaritycoefficientsetas0.96fortheclassification,alltestedvarietiesshowedtheiruniquespecificitybyclusteranalysis,whichindicatedthat24pairsofSSRcoreprimerswasabletoeffectivelyidentify123varietiesofrice.

2.2AFLPmarker

SixpairsofAFLPprimerswithrichpolymorphismwerescreenedbyLietc[22]toconductfingerprintinganalysisontwoChinesecabbagesamples（label587and586）aswellasastandardsample.Euclideandistancescoefficientofeachsamplewasestimated,indicatingthatdistinctdifferencewasfoundbetweenthesample587andstandardsample,withthepolymorphismbandrateof31.7%.Consequentlyvariety587wasidentifiedasadifferentvarietyfromthestandardsample.Inparison,variety586showedconsistentPCRbandswiththestandardsample,whichwasconsequentlyidentifiedasthesamevarietyasthestandardsample.ThisresearchdemonstratedthatAFLPwascapableofprovidingreliabledifferentiationtechnologyforplantcultivars.Intotal14samplesofeightvarietiesandsixwild

关于生物多样性论文范文集populationsofToxicodendronvernicifluumfromShaanxiwerechosenbyWeietc[23]forthedevelopmentofvarietyidentificationtechnique.BothmorphologicalandAFLPmolecularmarkerswereexaminedwith26morphologicalcharacterindexesand8AFLPprimers（EcoRⅠ+3/MseⅠ+3）.Multivariatestatisticanalysiswasconductedonmorphologicalmarkers,resultingin3principleponentindex（PCI）.ThefistPCIincludedtheratioofpetalandanther,lengthtowidthofthefifthlobular,thelengthanddiameteroffilament；thesecondPCIcoveredthelengthofpoundleafandpetioleofpoundleaf,thenumbersofleaflet,thefifthlobular,andthetoplobular；andthethirdPCIwerethetoplobularandthevertexangleofthefifthlobular,whichrespectivelycontributedto30.383%,19.321%and13.777%ofvarianceinmorphologyof14varieties.Furthermore,molecularmarkersof8AFLPprimers（EcoRⅠ+3/MseⅠ+3）alsopletelydistinguish14cultivars,inconsistencewithmorphologicalmarkers.

Wenetc[24]triedtodistinguish26jujubecultivarsand1sourjujubebyadoptingfluorescent-labeledAFLPmarkers.8AFLPprimerpairswerechosen,leadingto886AFLPmarkersidentifiedintotal.AmongtheseAFLPmarkers,112markerswereidentifiedasuniquebandsforspecificvarieties,whereas60markersweredeletionbandsforspecificvarieties,leadingtoeffectiveidentificationofjujubecultivars.

Songetc[25]chosen90cultivarsofChinesecabbagesfrom7differentproductionareas,anddevelopedfingerprintingtechniquebasedonAFLPmarkersfort

关于生物多样性论文范文集,与保护遗传学方法在生物多样性监测和评价领域的应用相关论文下载参考文献资料：