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heidentification.Intotal20pairsofAFLPprimersweredesignedtoexaminethegeicpolymorphismofthesecultivars,andAFLPprimersvariedbroadlyintermsofdifferentiationcapacityofChinesecabbagevarieties.ThenumberofpolymorphicbandsthatweredetectedbyAFLPprimersdifferedfrom9to32.Abinationofprimers（E-ACA/M-CTG）resultedin71amplifiedbands,including32polymorphicbands,whicheffectivelydistinguishedallofthe90varieties.Inparison,thegeicpolymorphismbetweenindividualsofthesamevarietywasalsoexaminedbyAFLPmarkertechnique.Twohybridcultivars（Beijingxin2andJingxiawang）ofChinesecabbagewereselectedand10individualswerechosenfromeachcultivar.TheAFLPbandsshowedconsistencebetweenindividualsofthesamevariety,exceptthatoneofBeijingxin2differedfromtheothers.2.3Capillaryelectrophoresiswithfluorescencedetection

Comparedwithpolyacrylamidegelelectrophoresisandsilverstainingtechnique,capillaryelectrophoresiswithfluorescencedetectionmethodismoreautomatedandprogrammed.Thesystemsoftwareofcapillaryelectrophoresiswithfluorescencedetectionisabletocalibratethedifferencesbetweencapillaryelectrophoresis,andreducetheartificialandsystematicerrors,whichconsequentlyimprovesthestabilityandrepeatabilityofvarietyidentificationtests[26].Fengetc[3]screened58SSRprimerstoidentify14Poplarvarietiesbyapplicationofcapillaryelectrophoresiswithfluorescencedetection,whichincluded4varietiesofPopulusdeltoids,5varietiesofPopulusnigra（including3transgenicvarieties）and4hybridvarieties.Theresultsshowedthatthe4varietiesofP.deltoids,5varietiesofP.nigra,and4hybridvarietieswereeffectivelyidentifiedby4primers,5primers,and4primersrespectively,withsignificantdifferenceobservedattheSSRlocibetweenP.deltoidesandP.nigra.DifferentSSRgenotypeswerealsoidentifiedbetweenthetransgenicandnon-transgenicvarieties.

3ConclusionandImplicationforBiodiversityMonitoringandAssessment

InparisontotheDNAmolecularmarker,cytologicalmarkertechniquesresultinlesspolymorphismforthesub-populations’differentiationofaplantspecies,butobviouslyreducethecostofthiswork,oncebiodiversitymonitoringandassessmentprojectsareimplementedatlargescale.Consequently,cytologicalmarkerwouldbemoresuitableasthemainsolutionforenvironmentalengineerstoconductgeicresourcecollectionwork,basedonwhichDNAmolecularmarkerwouldbeeaplementarysolution.Capillaryelectrophoresiswithfluorescencedetectionmethodcertainlyleadstohigheraccuracyandstabilityforidentificationtests.Nevertheless,therelativelycheaperfacilitiesrequiredbypolyacrylamidegelelectrophoresisandsilverstainingtechniquewouldbemoreacceptableinpractice,whichhasbeenadoptedbyrecentNationalStandardsincludingProtocolofPurityIdentificationforSoybeanVarietyusing-SSRMolecularMarkers（NY/T1788-2009）,aswellasGenuinenessandPurityVerificationofPotatoSeedTuber-SSRMolecularMarker（GB/T28660-2012）.

CollectionandstorageofsamplinglocationinformationaswellasphotosofplantmorphologicalcharactersareusuallynecessaryforthegeicresourcecollectionworkasindicatedbyRegulationfortheCollectionofGeicResources（HJ628-2011）,andGIStechnologyprovidesasupportivetoolforthecollectionandstorageofbothlocationinformationandfieldsamplingphotos[27]inthisprocess.【参考文献】

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