当前位置 —论文—写论文— 范文

关于统计学相关论文范文集,与自考本科毕业文范文相关论文参考文献格式

本论文是一篇关于统计学相关论文参考文献格式,关于自考本科毕业文范文相关研究生毕业论文开题报告范文。免费优秀的关于统计学及论文及统计分析方面论文范文资料,适合统计学论文写作的大学硕士及本科毕业论文开题报告范文和学术职称论文参考文献下载。

mPopulations:theSalar,Bo'anandDongxiang"的文章为例,分析研究方法的撰写.

Ethicalapproval

ThestudyoftheChineseMuslimmunitieswasperformedwiththeapprovaloftheEthicsCommitteeofTongjiMedicalUniversity,PRChinaandEdithCowanUniversity,Australia.PriorconsenttoundertaketheinvestigationwasobtainedatmunitylevelthroughthecooperationoftheChineseAcademyofSciences,Beijing.

该段说明了本研究考虑到了有关伦理学的问题强调在进行研究之前已经得到了伦理委员会的支持并与研究者签署了知情同意书.

Analyticalmethods

Finger-prickbloodsamplesfrom81Salar(52males,29females),67Bo'an(47males,20females)and64Dongxiang(49males,15females)wereobtainedfromrandomlyselectedindividualsineachmunity,allofwhomwereresidentinJishisanCounty,aminorityautonomousregionlocatedinGansuprovince,northwestChina.Theoverallagerangeofthesubjectswas12to18years.

本段介绍了研究对象的选取与分组情况,及其处理方法.

AsetoftendinucleotideSTRmarkersonchromosomes13and15(D13S126,D13S133,D13S192,D13S270,D15S11,D15S97,D15S98,D15S101,D15S108andGABRB3)wasinvestigatedinallsamplesfollowingprocedurespreviouslystandardizedonEastandSouthAsianpopulations.ForY-chromosomeanalysis,onetri-andthreetetranucleotideSTRmarkers(DYS19,DYS388,DYS389I,DYS393)werechosenfromapanelofmarkerswidelyusedinforensicexaminations.PCRconditionsfortheautosomalmarkersandfortheY-chromosomemarkerswereaspreviouslydescribed(Wangetal.2000).SeparationofthePCRproductswasperformedon6%denaturingpolyacrylamidegelsusinganABI373Aautomaticsequencer(AppliedBiosystems).TheGenotyperprogramwasusedtosizeSTRallelesbyreferencetoaninternalsizestandardGenescan-500TAMRA(AppliedBiosystems).

本段介绍了应用ABI373ADNA测序仪检测常染色体和Y染色体的STR位点.

Malesampleswereanalyzedfor15uniqueeventpolymorphisms(UEPs)ontheY-chromosome:M1(YAP),M216/M130(RPS4Y),M89/M213,M172,M170,M9,M175/M214,M122,M134,M159,M119,M95,M45,M173,andM17(Underhilletal.2001).PCRprimerswereasdescribedelsewhere(Underhilletal.2001),withtheexceptionoftheforwardprimersforM45(5(-ATTGGCAGTGAAAAATTATAGCTA-3()andM17(5(-GTGGTTGCTGGTTGTTACGTG-3(),whichweredesignedwitha3(mismatchcreatingarestrictionfragmentlengthpolymorphism(RFLP)site.M45wastypedbyBfaIrestrictiondigestionofthePCRproductandM17byAflIIIdigestion.FiveothermarkersweretypedusingPCR-RFLPassaysaccordingtothemanufacturer'sinstructions(NewEnglandBioLabs):M130(BslI),M213(NlaIII),M9(HinfI),M175(MboII),andM122(NlaIII).M1wasdetectedbyPCRamplificationofeithera455-bp(YAP+)or150-bp(YAP()fragmentthatcanberesolvedbyelectrophoresison2%agarose.Theremainingpolymorphismswereanalysedusingamodifiedversionoftheprimer-extensionassay(protocolavailableonrequest)andmatrix-assistedlaserdesorption/ionizationmassspectrometry(HaffandSmirnov1997).MassspectrawerecollectedusingaVoyager-DEPROMALDI-TOFinstrument(AppliedBiosystems).Genotypesweredeterminedbycalculatingthemassofthedideoxynucleotideincorporatedatthevariantsite.

本段介绍了应用飞行质谱检测Y-染色体基因多态性.

ThemtDNAhypervariableregionI(HV-I)wasamplifiedandsequencedinasubsetof30samples(n等于10fromeachmunity)accordingtoHopgoodetal.(1992),usingABIPrismdyeprimerkitsrunningon6%denaturingpolyacrylamidegels.SequencedatawereanalyzedusingABIDNAanalysissoftwareandSEQUENCER(GeneCodes).

本段介绍了线粒体DNA的检测.

以上三段介绍了研究测量的指标,数据收集和测量的方法,详细地描述了该项研究的实验流程.

Statisticalmethods

Fortheautosomaldata,basicstatisticalputationsincludedallelefrequency,heterozygosityandgenediversity,withHardy-Weinbergequilibrium(HWE)testsperformedusingtheGENEPOPprogram.AnexactprobabilitytestwasemployedtoassessthesignificanceofdeviationfromHWE.WheredeviationfromHWEwasconfirmed,aU-testwasusedtofurtherassesswhetheritwasduetoheterozygotedeficiency.BoththeexactprobabilitytestandtheU-TestarebasedonMarkovchainMonteCarlotypealgorithms.Thecorrelationofgenesofindividualswithinpopulations(FIS)wascalculatedforeachpopulation.

该段介绍了本研究所使用的统计学方法.

Thedegreeofpopulationdifferentiationfortheautosomal,Y-chromosomeandmtDNAdatasetswasascertainedbyanalysisofmolecularvariance(AMOVA),calculatedusingArlequin.Nei'sstandarddistance(Ds)wascalculatedforautosomalandY-chromosomeSTRdatausingtheMicrosatsoftwarepackage.TamuraandNei'sdistancewascalculatedforthemtDNAdatausingMega2.1.ThisprogramwasalsousedtogenerateNeighbour-Joining(NJ)phylogeictreesfromtheautosomal,Y-chromosomeSTRsandmtDNAdistancematrices.Thestatisticalrobustnessofthetreeswastestedonaparisonof1000bootstrapiterations.

本段介绍该项研究所采用的统计学方法及进行各种统计学分析所使用的软件.