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1;同根系土壤在取样期内各微生物PLFAs平均含量,计算植物疫苗胁迫处理后不同作物根系土壤各类微生物PLFAs平均含量增加或降低百分比,并作图,结果见图5-1-5.与对照组(CK)相比,工程菌FJAT-1458处理后,可将脂肪酸生物标记的变化分为类,第1类为下降型,16:0,19:0,18:0,16:1ω5c,i17:0,a16:0等脂肪酸标记18:1w9c含量降低达45.57%和81.14%.茄子,番茄,芹菜根系土壤微生物PLFAs组成16:0含量降低最大达到398.14%该标记指示假单胞菌辣椒,丝瓜根系土壤微生物PLFAs组成19:0含量降低最大关于生态学报文模板资源科学的毕业论文开题报告范文

微生物相关论文范文参考文献;达到294.43%该标记指示细菌.第2类为无变化型,其中茄子根系土壤脂肪酸包含11:0,i14:0,17:03OH等脂肪酸标记,辣椒根系土壤脂肪酸包含a11:0,i18:1H,i16:1G,16:1w11c,i14:1E,18:1ω6c等脂肪酸标记,丝瓜根系土壤脂肪酸包含a17:1B,i11:0,17:03OH,i12:0,18:12OH,10Me18:0等脂肪酸标记,番茄根系土壤脂肪酸a11:0,i18:1H,i12:03OH,i19:1I,i16:1G,i14:03OH,17:03OH,18:1ω6c等脂肪酸标记,芹菜根系土壤脂肪酸包含a11:0,16:02OH,i12:03OH,i14:0,i16:03OH,16:0Nalcohol等脂肪酸标记,均指示细菌,第3类为增加型茄子根系土壤脂肪酸包含16:02OH,a17:1B,a15:1A,16:1w11c,18:3w6c15:0,18:2w6,9c,i16:0,a17:0,a15:0等脂肪酸标记,辣椒根系土壤脂肪酸包含i11:0,11:03OH,i20:0,20:1ω9c,17:03OH,i14:0a17:0,i16:0,a15:0,18:2w6,9c,18:1w9c等脂肪酸标记,丝瓜根系土壤脂肪酸包含a17:1B,i11:0,16:02OH,cy17:0,10Me18:0i15:0,a17:0,a17:0,18:2w6,9c,i16:0,a15:0等脂肪酸标记,番茄根系土壤脂肪酸包含a11:0,i18:1H,i12:03OH,i19:1,i16:1G,i14:03OH,17:03OH,18:1ω6ca17:0,i15:0,i16:0,a15:0等脂肪酸标记,芹菜根系土壤脂肪酸包含17:1ω8c,17:0,17:03OH,10Me18:0,i15:03OH,i16:1Hi15:0,a17:0,i16:0,a15:0等脂肪酸标记10Me17:0,10Me18:0,该标记平均含量增加图5-1FJAT-1458胁迫下茄子根系土壤微生物PLFAs含量变化

Fig.5-1ChangofPLFAscontentineggplantrhizospheresoilbystrainFJAT-1458treatmenting

图5-2FJAT-1458胁迫下辣椒根系土壤微生物PLFAs含量变化

Fig.5-2ChangofPLFAscontentinpepperrhizospheresoilbystrainFJAT-1458treatmenting

图5-3FJAT-1458胁迫下丝瓜根系土壤微生物PLFAs含量变化

Fig.5-3ChangofPLFAscontentinloofahrhizospheresoilbystrainFJAT-1458treatmenting

图5-4FJAT-1458胁迫下番茄根系土壤微生物PLFAs含量变化

Fig.5-4ChangofPLFAscontentintomatorhizospheresoilbystrainFJAT-1458treatmenting

图5-5FJAT-1458胁迫下芹菜根系土壤微生物PLFAs含量变化

Fig.5-5ChangofPLFAscontentinceleryrhizospheresoilbystrainFJAT-1458treatmenting

以不同作物根系土壤各类微生物PLFAs平均含量5-6.当兰氏距离为48.63时,可分为3大类群.PLFAs平均含量PLFAs平均含量图5-6不同作物根系土壤各类微生物PLFAs平均含量聚类分析

Fig.5-ClusterananlysisofmicrobialPLFAsaveragecontentinthedifferentcroprhizospheresoil

5.3.3植物疫苗胁迫下不同作物根系土壤微生物群落结构变化动态

比较植物疫苗工程菌FJAT-1458处理后根系土壤微生物量的变化特征(表5-2),结果表明,工程菌FJAT-1458处理后的不同时期,不同作物根系土壤微生物总量均比对照组高,说明无致病力青枯雷尔氏菌处理能够富集根系土壤的微生物.不同不同时间的比较PLFA总量呈,步分析发现,工程菌FJAT-1458处理能够促进作物根系土壤细菌和真菌的生长,而抑制放线菌的生长.

表5-2菌株FJAT-1458处理对不同作物根系土壤微生物PLFA含量影响(nmol﹒g-1)

Tab.5-2EffectofstainFJAT-1458onmicrobialPLFAscontentindifferentcroprhizospheresoil

不同作物根系土壤处理时间(d)处理组对照组总微生物量细菌Bacteriaingeneral真菌Fungi放线菌Actinomycete总微生物量细菌Bacteriaingeneral真菌Fungi放线菌Actinomycete茄子根系土壤332.0510.932.8944.6632.746.944.98645.6534.317.853.4945.1434.276.094.78945.9934.717.244.0445.4132.957.215.251246.9336.236.674.0344.2133.526.064.631547.6436.587.024.0445.6234.676.244.711848.8738.226.524.1347.3235.126.635.572144.5635.055.424.0944.8833.95.155.83辣椒根系土壤327.2517.152.1845.0633.376.914.78647.9430.5614.582.845.2134.275.855.09948.532.3612.633.5143.533.225.694.591247.8434.539.653.6642.1132.585.274.261546.9634.798.154.0247.8337.085.764.991846.6234.488.173.9746.4635.75.884.882145.1233.427.334.3740.8131.414.724.68丝瓜根系土壤333.817.913.2244.1131.827.594.70645.3533.447.854.0643.6832.456.664.57945.6334.17.164.3745.5234.146.774.611245.1334.466.434.2451.0938.697.844.561547.6536.16.664.8948.7937.016.675.11184634.816.644.5544.1633.745.704.722145.135.886.083.1442.2232.525.184.52番茄根系土壤333.976.484.0143.2531.816.484.96644.4934.525.654.3242.531.566.194.75945.6235.675.554.445.2334.086.214.941246.0835.765.544.7842.8832.475.934.481547.1436.435.774.9445.2634.186.114.971848.0337.395.595.0548.0236.86.195.032147.5837.864.675.0543.6333.15.335.20芹菜根系土壤334.366.563.5344.5131.188.434.90645.135.545.713.8544.3331.947.365.03945.1734.756.194.2343.2231.816.564.851246.236.275.414.5244.5132.966.614.941546.5135.926.044.5545.233.596.595.021847.2336.226.164.8543.8332.66.264.972147.136.36.144.6646.1233.986.086.06

5.3.4植物疫苗胁迫下不同作物根系土壤微生物群落结构多样性分析

多样性指数在评价土壤的微生物群落多样性时非常有效,高的多样性指数值表明高的微生物群落多样性[21].Pielou指数反表为植物疫苗工程菌FJAT-1458处理对根系土壤微生物群落多样性指数的影响.植物疫苗工程菌FJAT-1458处理对作物根系土壤微

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