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遗传学方面论文范例,与畜牧兽医专业相关毕业论文模板

本论文是一篇遗传学方面毕业论文模板,关于畜牧兽医专业相关在职研究生毕业论文范文。免费优秀的关于遗传学及中国畜牧兽医及动物方面论文范文资料,适合遗传学论文写作的大学硕士及本科毕业论文开题报告范文和学术职称论文参考文献下载。

更多地在氨基酸水平上表现,这与罗庆斌等(2005),Liu等(2002)以及Lima-Rosa等(2004)的研究结果相符合.当然所有的这些研究结果都还是初步的,还有待更深入的研究和探讨,以便进一步的搞清楚MHC与疾病抗性的关系,并使之运用于实际的育种工作之中.

鸡,MHC-B-F基因,外显子3,遗传变异

SystemsAnalysisofEpigeicandGeicEffectsonImmuneResponse

GenesinSusceptibilitytoMarek'sDiseaseofChickens

YingYu12,ApratimMitra1,ShuangChang3,FeiTian1,XiaoliXie1,YuanZhang2,

HuanminZhang3,JiuzhouSong1

(1.DepartmentofAnimal&,AvianSciences,UniversityofMaryland,CollegePark,MD20742,USA,2.LabofAnimalBreedingandGeics,CollegeofAnimalSciencesandTechnology,ChinaAgriculturalUniversity,Beijing100193,P.R.China.,3.USDA-ARS,AvianDiseaseandOncologyLaboratory,

EastLansing,MI48823,USA.)

INTRODUCTION

ChickenMarek'sdisease(MD)isavirus-inducedTcelllymphomaandcausedbyoncogenicherpesvirus,Marek'sdiseasevirus(MDV).HighlyinbredWhiteLeghornlines,MD-resistantline63and-susceptibleline72,areidealmodelstostudythemechanismsofviral-relatedtumorresistanceinchickens.

MATERIALSANDMETHODS

Lines63and72havebeendevelopedandmaintainedsince1939inAvianDiseaseandOncologyLaboratory.SpleentissuesoffourchickensforeachlinewerecollectedatADOLat5dayspostinfection(DPI),10DPIand21DPI.MDVuninfectedchickensateachtimepointwereservedascontrol.WeanalyzedDNAmethylationvariationsandDNAmutationsofmethyltransferasegeneDNMTsaswellasgenome-widetranscriptionalexpressionsinthespleentissueofthetwolinesbybisulfitepyrosequencingtechniqueandmicroarrayassays(Agilent4x44Kchickenmicroarray).DataadjustmentandstatisticallysignificantexpressedgeneswasperformedusingLimmapackage.

RESULTS

WiththecriterionoflogFC(foldchange)>,1.5andofPvalue<,0.01,morethan1000significantlydifferentiallyexpressedgeneswereidentifiedbetweenMDV-infectedline63and72birdsat21DPI.Mostofthemaretranscriptsofimmune-responserelatedgenes.Withpathwayanalysissoftware,wefoundthattheprocessofantimicrobialresponsewasresponsibleforMDresistanceinline63,whiletheprocessesofimmuneresponse,cancerandcelldeathcontributedtotheMDdevelopmentinline72.760significantlydifferentiallyexpressedtranscriptswerefoundbetweentheMDVnon-infectedline63and72at21DPI.TheresultindicatedthatthereweredistinctdifferencesofgeicresistancetoMDbetweentwolines.Moreover,wedisclosedsignificantlyincreasedDNAmethylationlevelsonthefirstexonofDNMT3ainMDVinfectedline63and72paredtotheuninfectedchickens(P<,0.05),whichwerenegativelycorrelatedwiththemRNAexpressionlevelsofDNMT3ainbothlines.ItisnotingthattwoCGtoTGmutationsonthefirstexonofDNMT3bwerediscoveredinline72paredtoline63,whichmaycontributetothedown-regulationofDNMT3binline72(P<,0.05).

DISCUSSIONANDCONCLUSION

WeprovidesystematicexperimentalevidencethattheexposureofMDVmarkedlyincreasedthegenesexpressionofimmune-response,cancerandcelldeathinMD-susceptibleline72,whileantimicrobialresponsegeneswereobviouslyenhancedinMD-resistantline63.Inaddition,thelowerexpressionofDNMT3aandDNMT3bmaydown-regulatethemethylationstatusofkeygenesinline72thenup-regulatetheexpressionlevelofthegenes.

本篇论文来源 http://www.sxsky.net/xie/070675786.html

KEYWORDS:chicken,Marek'sdisease,immuneresponsegenes

ACKNOWLEDGMENT

TheabstractwasacceptedbyinternationalconferenceofEpigeics,DevelopmentandHumanDiseaseonJanuary5-10,2016inColorado,USA.TheworkwassupportedbytheNational"948"project(2006-G48)ofChinaandUSDA-NRI2016-00870ofUSA.